SO WHY IS GFP THE BEST?
- Well, GFPs are these awesome green colored proteins...
And...
- They have been successfully engaged in virtually EVERY biological field, from microbiology to human physiology.
- These ubiquitous markers are extremely useful as reporters for gene expressions in cultured cells, excised tissues, and whole animals.
- In live cells, GFPs are mostly used to track the localization and dynamics of proteins, organelles, and other cellular compartments.
- They can also be used to assess protein-protein interactions through the use of "resonance energy transfer techniques (FRET )."
- The application of GFPs consists of fusing the gene for the cDNA of the target gene to that of a GFP followed by transferring the resulting recombinant vector into a host cell or whole organism.
- "Light is produced by the bioluminescent jellyfish Aequorea victoria when calcium binds to the photoprotein aequorin. This jellyfish produces green lights. This light is the result of a second protein in A. victoria that derives its excitation energy from aequorin, the green fluorescent protein." (http://pubs.acs.org/doi/pdf/10.1021/bi00056a003)
- "Purified GFP, a protein of 238 amino acids, absorbs blue light (maximally at 395 nm with a minor peak at 470 nm) and emits green light (peak emission at 509 nm with a shoulder at 540 nm)" (http://pubs.acs.org/doi/pdf/10.1021/bi00056a003)
- It provides an excellent means for monitoring gene expression and protein localization in living cells.
- Because it does not appear to interfere with cell growth and function, GFPs are also a convenient indicator of transformation and one that allow normal cells to be separated with fluorescence-activated cell.
- GFP can be used as a vital marker so that cell growth and movement can be followed, especially in animals that are essentially transparent like C. elegans and zebra fish.
- The relatively small size of the protein my facilitate its diffusion throughout the cytoplasm of extensively branched cells like neurons and glia.
http://zeiss-campus.magnet.fsu.edu/articles/probes/fpconsiderations.html |
Pymol; protein data bank 1S6Z |
Pymol; protein data bank 1S6Z |
Pymol; protein data bank 1S6Z |
- GFP has jumped from being insignificant to becoming one of the most widely studied and used proteins in biochemistry and cell biology.
- It is very fascinating and amazing to observe GFP's ability to emit an internal flurophore
- GFP has a high resolution structure that helps to understand the relation between protein structure and spectroscopic function.
- GFP is used extensively in observing protein interaction and gene expression in intact cells and organisms
- GFP are also used to find out the behaviors of cancerous cells, which is a huge contribution to the field of oncology in the discovery of new type of cancers.
I'm sure you're convinced by now that GFPs are the best :)
References
http://zeiss-campus.magnet.fsu.edu/articles/probes/fpconsiderations.html
http://www.ncbi.nlm.nih.gov/pubmed/9759496
http://www.tsienlab.ucsd.edu/Publications/Ormo%201996%20Science%20-
%20Crystal%20structure.PDF
http://119.93.223.179/ScienceDirect/Current%20Biology/06-02/sdarticle_022.pdf
http://pubs.acs.org/doi/pdf/10.1021/bi00056a003
What sets this blog apart is that you are deliberately showing why your protein is the best, instead of finding information and making your readers fish through the information.GFP is a simple protein, but is used to in so many fields of science that it is more or less irreplaceable, and in a way deserves to honored at least once as the protein of the year.As for the images, maybe a space filling model would be a good addition to the gallery.
ReplyDeleteAll the facts and information about GFP would draw enough interest from people in scientific field. I think It would be more interesting if there was more specific explanation on biochemistry or structure of GFP. But it was a good time looking at some picture of the protein that I have heard a lot.
ReplyDeleteYou did a great job o showing right off the bat the importance of your protein and specifically what distinguishes it from many other proteins. I really like the bullet point aspect of the journal highlighting the most important aspects of the protein. I would say however that i think a sphere modelling of the protein would be neat to see and looking at the other models it looks like there is a hollow area in the middle maybe? If so you might want to create another model showing off the unique structure of the protein, drawing to its tube like shape.
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